E-MTAB-3798 - Time course Chromatin Immunoprecipitation on Drosophila virilis embryos during embryogenesis

Status
Last updated on 31 May 2017, released on 1 October 2017
Organism
Drosophila virilis
Samples (40)
Protocols (10)
Description
ChIP followed by next generation sequencing over 5 developmental time points of Drosophila virilis embryos (w[-], white eye mutation line) against 5 key mesodermal factors (Twist, Tinman, Mef2, Bagpipe and Biniou) were performed. The aim was to compare binding profiles of these 5 mesodermal factors between two Drosophila species, D. melanogaster (Zinzen et al., 2009) and D. virilis (this study). D. virilis specific antibodies were used for this purpose for 4 of the 5 factors (D. melanogaster Anti-body for Mef2 showed high specificity in D. virilis). Two biological replicates for each condition were sequenced using Illumina HiSeq.
Experiment types
ChIP-seq, binding site identification design, development or differentiation design, replicate design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-3798.idf.txt
Sample and data relationshipE-MTAB-3798.sdrf.txt
Links