E-MTAB-376 - ChIP-Seq of six Drosophila species with anti-Drosophila melanogaster Twist and anti-Snail antibodies using different library fragment sizes
Released on 7 December 2010, last updated on 24 October 2013
Drosophila ananassae, Drosophila erecta, Drosophila melanogaster, Drosophila pseudoobscura, Drosophila simulans, Drosophila yakuba
The binding patterns of some transcription factors have been shown to diverge substantially between closely related species. Here, we show that the binding pattern of the developmental transcription factor Twist is highly conserved across six Drosophila species, revealing strong functional constraints at developmental enhancers. Conserved binding correlates with sequence motifs for Twist and its partners, permitting the de novo discovery of their cooperative binding. It also includes over 10,000 low-occupancy sites near the detection limit, which tend to mark enhancers of later developmental stages. We predict that conservation, dynamic occupancy, and combinatorial regulation will be generally true for developmental enhancers.
ChIP-seq, binding site identification, replicate, species
Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. :2705-2713 (2013), PMID:23980024