E-MTAB-3712 - Identification of chicken Interferon Stimulated Genes (ISGs) using Affymetrix Chicken Gene 1.0 ST Arrays
Submitted on 10 July 2014, last updated on 1 September 2015, released on 1 December 2015
Recombinant chicken IFN1 was prepared as previously reported (Laidlaw et al, 2013) and was added in chicken embryo fibroblasts to a final concentration of 1000 U/ml. Confluent cells were treated with chicken IFNα or mock treated and incubated for six hours before harvesting. The experiment was repeated in triplicate with three different batches of CEFs.
transcription profiling by array, compound treatment design, reference design, replicate design
Chicken interferome: avian interferon-stimulated genes identified by microarray and RNA-seq of primary chick embryo fibroblasts treated with a chicken type I interferon (IFN-α). Giotis ES, Robey RC, Skinner NG, Tomlinson CD, Goodbourn S, Skinner MA. :75 (2016), PMID:27494935