5 protocols
array scanning and feature extraction protocol
nucleic acid hybridization to array protocol
nucleic acid labeling protocol
3'-IVT Express (Affymetrix)
growth protocol
cell lines cultured in RPMI or DMEM/F12 medium and collected as flash-frozen pellets when growing in log phase
nucleic acid extraction protocol
RNeasy Mini kit (Qiagen) followed by DNase digestion in solution, Qiagen RNeasy cleanup and elution