E-MTAB-3543 - Th2 differentiation in a time course experiment and at single generation resolution
Released on 1 April 2016, last updated on 14 April 2016
Mouse splenic naïve T cells were activated (with anti-CD3/CD28)and polarized towards the Th2 subtype with IL4 . Cells were collected at different time points during the differentiation and, at day 3 .5,were collected according to number of cell divisions and expression or absence of a specific differentiation marker.
RNA-seq of coding RNA, cell type comparison design
Single-cell analysis of CD4+ T-cell differentiation reveals three major cell states and progressive acceleration of proliferation. Proserpio V, Piccolo A, Haim-Vilmovsky L, Kar G, Lönnberg T, Svensson V, Pramanik J, Natarajan KN, Zhai W, Zhang X, Donati G, Kayikci M, Kotar J, McKenzie AN, Montandon R, Billker O, Woodhouse S, Cicuta P, Nicodemi M, Teichmann SA. :103 (2016), PMID:27176874