E-MTAB-3543 - Th2 differentiation in a time course experiment and at single generation resolution

Status
Released on 1 April 2016, last updated on 14 April 2016
Organism
Mus musculus
Samples (20)
Protocols (8)
Description
Mouse splenic naïve T cells were activated (with anti-CD3/CD28)and polarized towards the Th2 subtype with IL4 . Cells were collected at different time points during the differentiation and, at day 3 .5,were collected according to number of cell divisions and expression or absence of a specific differentiation marker.
Experiment types
RNA-seq of coding RNA, cell type comparison design
Contacts
Citation
Single-cell analysis of CD4+ T-cell differentiation reveals three major cell states and progressive acceleration of proliferation. Proserpio V, Piccolo A, Haim-Vilmovsky L, Kar G, Lönnberg T, Svensson V, Pramanik J, Natarajan KN, Zhai W, Zhang X, Donati G, Kayikci M, Kotar J, McKenzie AN, Montandon R, Billker O, Woodhouse S, Cicuta P, Nicodemi M, Teichmann SA. :103 (2016), PMID:27176874
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-3543.idf.txt
Sample and data relationshipE-MTAB-3543.sdrf.txt
Processed data (1)E-MTAB-3543.processed.1.zip
Links