E-MTAB-3478 - A Drosophila chitinase-like protein has an immune function
Submitted on 1 December 2011, last updated on 20 January 2016, released on 20 January 2016
In the present study we analyzed the function of one member of Drosophila CLPs namely Drosophila IDGF3 with a special focus on immunity. We found that Idgf3 mutants are homozygous viable, and have defects in hemolymph clotting, which is the earliest of Drosophila larvae response after injury. We could further demonstrate that IDGF3 contributes to fly immunity: idgf3 mutants show increased sensitivity to Gram-negative bacteria as well as increased mortality after nematode infections. IDGF3 overexpression leads to a hyper-coagulation phenotype in larvae and a decrease in viability of adult flies. Transcription profiling further confirmed that IDGF3 is involved in the activation of innate defense mechanisms and signal pathways connected to wound healing and regenerative processes. A large fraction of immune- and regenerative genes require IDGF3 for their induction, suggesting that ΓÇô similar to Chi3l1- IDGF3 is a key regulators of the epithelial response to injury and infection.
transcription profiling by array, disease state design