E-MTAB-3449 - Dose response of the BET inhibitor JQ1 in Small-Cell Lung Cancer cell lines
Submitted on 29 October 2013, released on 31 May 2015, last updated on 4 March 2016
This is a Dose response experiment carried out using the BET inhibitor JQ1 in SCLC cell lines. Approximately 1x106 cells of cell lines, DMS-53, H69, H1694, H1048, H2227 were treated for 2 hours with a 12 point dose range of JQ1 (7500nM to 0.04nM). Lysates were collected at the end of the treatment in 300ul TRI reagent for RNA preparation for expression profiling.
transcription profiling by array, cell type comparison design, compound treatment design, dose response design
Sensitivity of Small Cell Lung Cancer to BET Inhibition Is Mediated by Regulation of ASCL1 Gene Expression. Lenhart R1, Kirov S1, Desilva H1, Cao J1, Lei M1, Johnston K1, Peterson R1, Schweizer L1, Purandare A1, Ross-Macdonald P1, Fairchild C1, Wong T1, Wee S2. , PMID:26253517