E-MTAB-3352 - Transcriptional profiling of Ngn3-dependent pancreatic endocrine differentiation at E15.5

Status
Last updated on 10 July 2015, released on 1 October 2015
Organism
Mus musculus
Samples (6)
Protocols (6)
Description
This experiment used RNA-Seq technology to explore gene expression in mouse Ngn3^GFP/+ [het] FACS sorted pancreatic cells at E15.5 (commited endocrine progenitor cells) and in Ngn3^GFP/GFP [null] at E15.5 (defective endocrine progenitor cells). This experiment is designed to understand the gene expression alteration in the endocrine lineage at different embryonic days. The aim is to understand both Ngn3 dependent and independent gene expression profiles so as to reveal the instructive signals that specfy the collective endocrine islet cell fate or specific islet cell type.
Experiment types
RNA-seq of coding RNA, cell type comparison design, genetic modification design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
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