E-MTAB-3273 - Identification of a blood-borne miRNA signature of synovial sarcoma

Status
Submitted on 30 June 2014, last updated on 3 August 2015, released on 3 August 2015
Organism
Homo sapiens
Samples (10)
Array (1)
Protocols (6)
Description
Synovial sarcomas account for approximately 10% of all soft-tissue tumors and occur most frequently in young adults. A specific translocation in this sarcoma induces fusion of the SYT gene on chromosome 18 to the SSX genes on chromosome X, leading to proliferation of the tumor cells. The need for non-invasive biomarkers indicating recurrence and activity of this disease has sparked research into short non-coding RNA known as microRNA (miRNA). Patients and Methods: Blood samples of patients with active synovial sarcoma and of healthy donors were collected. Whole blood RNA was extracted and analyzed using an Affymetrix GeneChip miRNA Array v. 4.0. Results: Hierarchical clustering of all covered human mature miRNAs and human pre-miRNAs separated sarcoma samples from control samples. Following hierarchical clustering, the array results were narrowed own to deregulated miRNAs with a fold change > |3.0|, a p-value <0.005, a q-value (FDR-corrected p-value) <0.2 and a similar deregulation of related miRNAs with the same base sequence throughout the array. Unsupervised hierarchical clustering of the panel of miRNAs that met these criteria could again separate sarcoma patients from healthy controls. Conclusion: Our results have identified a specific whole blood miRNA signature that may serve as an independent biomarker for the diagnosis of local recurrence or distant metastasis of synovial sarcoma.
Experiment types
transcription profiling by array, case control design
Contacts
Citation
Identification of a blood-borne miRNA signature of synovial sarcoma. Fricke A, Ullrich PV, Heinz J, Pfeifer D, Scholber J, Herget GW, Hauschild O, Bronsert P, Stark GB, Bannasch H, Eisenhardt SU, Braig D. :151 (2015), PMID:26250552
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-3273.idf.txt
Sample and data relationshipE-MTAB-3273.sdrf.txt
Raw data (1)E-MTAB-3273.raw.1.zip
Processed data (1)E-MTAB-3273.processed.1.zip
Array designA-MTAB-537.adf.txt
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