E-MTAB-3198 - Regulation of the ESC transcriptome by nuclear long non-coding RNAs
Submitted on 26 December 2014, released on 1 December 2015, last updated on 9 January 2017
We performed poly(A)+ RNA sequencing (Illumina GAIIx) from nuclear and whole cell RNA fractions of mouse AB2.2 embryonic stem cells (ESCs) and neural progenitors (NPCs) differentiated from ESCs. We further transfected AB2.2 ESCs with control antisense oligonucleotides (ASOs), or 2 independent ASOs each targeting either PAT-14 lncRNA or Firre lncRNA for 24 hours, in 4 biological replicates each and performed poly(A)+ RNA sequencing (Illumina HiSeq 2000).
RNA-seq of coding RNA, cell component comparison design, cell type comparison design, cellular modification design, development or differentiation design
Regulation of the ESC transcriptome by nuclear long non-coding RNAs. Jan H. Bergmann, Jinging Li, Melanie A. Eckersley-Maslin, Frank Rigo, Susan Freier, David L. Spector.