E-MTAB-3132 - Detection of differential gene expression in locust brain infected with the protozoan parasite Neospora caninum using the drosophila gene chip
Submitted on 3 December 2013, last updated on 28 November 2014, released on 28 January 2015
To obtain a broader understanding of the effects of the N. caninum on S. gregaria adults, we examined the parasite impact on host gene expression using Affymetrix DNA microarray and Cross Species Hybridisation (CSH) analysis. Since N. caninum infection induced neurological injuries in locusts, it was important to test whether N. caninum infection had altered gene expression within locust brains. The analysis was designed to gain further understanding of the mechanisms for N. caninum neuropathy, and in particular, of the genes responsible for the capacity of N. caninum to establish brain infection. Using a cross species hybridisation (CSH) approach, RNA samples from brains of mock- and N. caninum-infected locusts were labeled and hybridised to Affymetrix Gene Chip Drosophila Genome 2.0 Arrays. Data were analysed using Partek Genomics Suite Version 6.4. Of the 28,593 transcripts represented on the GeneChip, approximately 18,500 were expressed in the N. caninum-infected locust’s brain. PCA quality control analysis shows that the uninfected and infected samples were biologically noisy but generally well separated .
transcription profiling by array, disease state design
Susceptibility to experimental infection of the invertebrate locusts (Schistocerca gregaria) with the apicomplexan parasite Neospora caninum. Mamdowh M. Alkurashi, Sean T. May, Kenny Kong, Jaume Bacardit, David Haig, Hany M. Elsheikha. PeerJ :e674 (2014)