E-MTAB-3126 - ChIP-Chip and SACO Analysis of Pdx-1 binding in NIT-1 Insulinoma Cells
Released on 21 November 2014, last updated on 25 November 2014
The BCBC Promoter Chip 5B was used to identify genomic targets of Pdx-1 binding. Genomic DNA from mouse NIT-1 insulinoma cells was immunoprecipitated with a Pdx-1 Antibody, PCR amplified, and labeled to the chip. All hybridizations were versus a common reference sample which was a labeled IgG IP. Additionally a Pdx1 SACO library for NIT-1 cells was generated.
ChIP-seq, ChIP-chip by array, binding site identification design, compound treatment design, reference design
Characterization of pancreatic transcription factor Pdx-1 binding sites using promoter microarray and serial analysis of chromatin occupancy. Keller DM, McWeeney S, Arsenlis A, Drouin J, Wright CV, Wang H, Wollheim CB, White P, Kaestner KH, Goodman RH.