E-MTAB-3050 - Microarray analysis of in vitro differentiation of adult human pancreatic progenitor cells

Status
Last updated on 30 October 2014, released on 31 October 2014
Organism
Homo sapiens
Samples (2)
Array (1)
Protocols (10)
Description
We have developed an in vitro culture system that allows us to expand progenitor cells from human islet preparations and differentiate them into insulin-producing cells. We noticed however, that cultures from individual islet preparations had very heterogeneous outcomes, from good differentiation to almost none. We therefore speculated that our progenitor cell cultures contained different kinds of cells and that the true endocrine progenitor cells are present in the successful cultures, but not in the unsuccessful ones. To address this issue and to begin to identify markers for the true endocrine progenitor cells we compared global gene expression between a very successful culture (final insulin-expression 10% of islets) and an unsuccessful one (final insulin-expression 0%). We also included RNA from freshly isolated islets for control purposes. The cultures from donor A yielded substantial differentiation, while the cultures from donor B showed no successful differentiation.
Experiment types
transcription profiling by array, cell type comparison design, development or differentiation design
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-3050.idf.txt
Sample and data relationshipE-MTAB-3050.sdrf.txt
Raw data (1)E-MTAB-3050.raw.1.zip
Processed data (1)E-MTAB-3050.processed.1.zip
Additional data (1)E-MTAB-3050.additional.1.zip
Array designA-AFFY-1.adf.txt
Links