E-MTAB-3042 - Transcription profiling by array of laser capture microdissected human islet beta cells
Last updated on 28 October 2014, released on 28 October 2014
Fresh frozen sections of islets obtained by surgery were laser capture microdissected using autofluorescence to guide selection of beta cell areas of the islet. RNA was extracted and amplified with 2 rounds of T7 linear amplification. Two technical replicates were hybridized to Affymetrix U95Av2 arrays.
transcription profiling by array, growth condition design, is expressed