E-MTAB-2904 - Collagen induces maturation of human monocyte-derived dendritic cells by signalling through Osteoclast Associated Receptor
Submitted on 30 March 2013, last updated on 9 September 2014, released on 31 October 2014
Human immature dendritic cells derived from monocytes were activated to become mature dendritic cells by exposure to platebound Collagen I or Collagen. The importance of OSCAR-signalling was evaluated by pre-incubating the immature dendritic cells with an inhibitory monoclonal antibody to the OSCAR receptor, or an isotype control. As a positive control, immature dendritic cells were activated by using LPS. The set-up was examined at 4 hours and 20 hours to evaluate early and late effects of OSCAR signalling.
transcription profiling by array, compound treatment design, time series design
Collagen induces maturation of human monocyte-derived dendritic cells by signalling through Osteoclast Associated Receptor. Schultz H.S., Nitze L.M., Zeuthen L.H., Keller P., Gruhler A., Pass J., Chen J., Demuth H., Guo L., Fleetwood A.J., Hamilton J.A., Berchtold M.W., Panina S.
Collagen induces maturation of human monocyte-derived dendritic cells by signaling through osteoclast-associated receptor. Schultz HS, Nitze LM, Zeuthen LH, Keller P, Gruhler A, Pass J, Chen J, Guo L, Fleetwood AJ, Hamilton JA, Berchtold MW, Panina S. :3169-3179 (2015), PMID:25725106