E-MTAB-2773 - Expression profiles of novel pancreatic cell lines
Last updated on 22 July 2014, released on 22 July 2014
We studied the expression profiles of two different cell lines that were generated in our laboratory from tumors found in mice expressing the SV40 T antigen under the control of the upstream glucokinase promoter. The first cell line, called GKP2 cells, was derived from an insulinoma and expresses insulin and pdx1 along with other markers of beta cells. The second cell line, named GKP4 cells, was derived from periductal tumors that did not express insulin or pdx1 but expressed genes of the endocrine lineage, such as Isl1 and Nkx2.2. Interstingly, a small fraction of the GKP4 cells can spontaneously turn on the expression of insulin and pdx1, indicating that they could be islet cell progenitors. The aim of our project was to identify the genes expressed specifically in GKP4 cells. To do so, we compared the gene expression profiles of GKP4 cells with GKP2 cells using DNA microarray.
transcription profiling by array, cell type comparison design, replicate design