E-MTAB-2641 - miRNA expression profile following Staphylococcal Enterotoxin B exposure in C3h/HeJ mice

Status
Submitted on 5 May 2012, last updated on 10 June 2014, released on 30 June 2014
Organism
Mus musculus
Samples (2)
Array (1)
Protocols (3)
Description
C3H/HeJ mice were either given a Dual Dose (5 ug intranasal and 2 ug intraperitoneal two hours apart) of Staphyloccocal entertoxin B (SEB) or PBS as a control for 72 hours in a Mice were sacrificed and lung infiltrating mononuclear cells were isolated. Total RNA extraction was performed using Qiagen miRNeasy kit and RNA quality was assessed specrophotometrically. Affymetric Gene Chip miRNA 3.0 array was used for miRNA profiling using FlashTag Biotin HSR RNA labeling kit.
Experiment types
microRNA profiling by array, disease state design
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-MTAB-2641.idf.txt
Sample and data relationshipE-MTAB-2641.sdrf.txt
Raw data (1)E-MTAB-2641.raw.1.zip
Array designA-AFFY-184.adf.txt
Links