E-MTAB-2641 - miRNA expression profile following Staphylococcal Enterotoxin B exposure in C3h/HeJ mice
Submitted on 5 May 2012, last updated on 10 June 2014, released on 30 June 2014
C3H/HeJ mice were either given a Dual Dose (5 ug intranasal and 2 ug intraperitoneal two hours apart) of Staphyloccocal entertoxin B (SEB) or PBS as a control for 72 hours in a Mice were sacrificed and lung infiltrating mononuclear cells were isolated. Total RNA extraction was performed using Qiagen miRNeasy kit and RNA quality was assessed specrophotometrically. Affymetric Gene Chip miRNA 3.0 array was used for miRNA profiling using FlashTag Biotin HSR RNA labeling kit.
microRNA profiling by array, disease state design
Role of miRNA in the regulation of inflammatory genes in staphylococcal enterotoxin B-induced acute inflammatory lung injury and mortality. Rao R, Nagarkatti P, Nagarkatti M. :284-297 (2015), Europe PMC 25564423