E-MTAB-2611 - MicroRNA profiling in four human bladder cancer cell lines
Last updated on 25 July 2014, released on 25 July 2014
To identify miRNA-target interactions that are important in bladder cancer metastasis, we profiled miRNA and mRNA expression in poorly metastatic cell lines T24 and LUC, and their metastatic derivatives FL4 and LUL2, respectively. We reasoned that miRNAs and genes that expressed differently between parental and derivative cell lines could potentially contribute to their disparity in metastatic competence. We further hypothesized that interactions between these miRNAs and target genes may play an important role in metastatic competence. We have developed the multiMiR R pacakge and database for such integrated analysis of miRNA-target interactions. Here we demonstrate how multiMiR was used to generate testable hypotheses that could be pursued experimentally. The data set here is from the miRNA expression profiling experiment. Please see E-MTAB-2610 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-2610/ ) for the mRNA profiling data set.
microRNA profiling by array, cell type comparison design, co-expression, in vitro
The multiMiR R package and database: integration of microRNA–target interactions along with their disease and drug associations. Yuanbin Ru, Katerina J. Kechris, Boris Tabakoff, Paula Hoffman, Richard A. Radcliffe, Russell Bowler, Spencer Mahaffey, Simona Rossi, George A. Calin, Lynne Bemis, and Dan Theodorescu. Nucleic Acids Research (2014)