E-MTAB-2580 - Effect of hypoxia on the Represser Element-1 Silencing Transcription factor (REST) in Human
Released on 17 August 2016, last updated on 18 August 2016
The Represser Element-1 Silencing Transcription factor (REST) was knocked-down in HEK293 cells using RNA intereference in cells exposed to 21% or 1% oxygen for 24 hours, the effects on transcript expression (poly(A)+ RNA)) were analyzed using genome wide sequencing (RNAseq).
RNA-seq of coding RNA, cellular modification design, in vitro
REST is a hypoxia-responsive transcriptional repressor. Miguel A. S. Cavadas, Marion Mesnieres, Bianca Crifo, Mario C. Manresa, Andrew C. Selfridge, Ciara E Keogh, Zsolt Fabian, Carsten C. Scholz, Karen A. Nolan, Liliane M. A. Rocha, Murtaza M. Tambuwala, Stuart Brown, Anita Wdowicz, Danielle Corbett, Keith J. Murphy, Catherine Godson, Eoin P. Cummins, Cormac T. Taylor, Alex Cheong. Scientific Reports 6(Aug 17):31355 (2016)