E-MTAB-2497 - RNA-seq of coding RNA from human HEK293-M2 cells with or without overexpression of RHOXF2 in the presence of mitomycin or DMSO

Status
Last updated on 15 April 2014, released on 15 May 2014
Organism
Homo sapiens
Samples (4)
Protocols (5)
Description
Overexpression of the transcription factor RHOXF2 in the HEK293_M2 cells confers resistance to several DNA damaging agents. To understand that resistance mechanism, we cultured HEK293_M2 with or without overexpression of RHOXF2 in the presence of 40nM of mitomycin C or DMSO (control) and profiled their transcriptional response by RNA-seq.
Experiment types
RNA-seq of coding RNA, co-expression, compound treatment design, genetic modification design, in vitro
Contact
Citations
A genome scale overexpression screen to reveal drug activity in human cells. Arnoldo A, Kittanakom S, Heisler LE, Mak AB, Shukalyuk AI, Torti D, Moffat J, Giaever G, Nislow C.
A genome scale overexpression screen to reveal drug activity in human cells. Anthony Arnoldo, Saranya Kittanakom, Lawrence E. Heisler, Anthony B. Mak, Andrey I. Shukalyuk, Dax Torti, Jason Moffat, Guri Giaever, and Corey Nislow.
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-2497.idf.txt
Sample and data relationshipE-MTAB-2497.sdrf.txt
Links