E-MTAB-2456 - PAT-seq - a high-throughput polyadenylation assay for the Illumina platform

Status
Last updated on 4 April 2014, released on 5 April 2014
Organism
Saccharomyces cerevisiae
Samples (16)
Protocols (4)
Description
Polyadenylation - the cleavage of the nascent mRNA and the addition of a tract consisting of adenine bases in a non-template-dependent manner - is a post-transcriptional modification characteristic of the majority of eukaryotic transcripts. In this study we explore PAT-seq, an Illumina-based poly(A) tail assay, based on the low-throughput G-tailing approach. Briefly, our approach is based on G-tailing poly(A) selected mRNA followed by fragmentation and reverse transcription using a mixture of primers targeting the poly(A)-tail/G-tag junction and the polyadenylation site and the body of the transcripts. The resulting cDNA fragments are sequenced using standard Illumina RNA-seq protocol.
Experiment types
RNA-seq of coding RNA, co-expression, replicate design, strain or line design
Contact
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-2456.idf.txt
Sample and data relationshipE-MTAB-2456.sdrf.txt
Processed data (17)Click to browse processed data
Links