E-MTAB-2301 - RNA-seq of coding RNA from 4-day old wheat root samples with or without colonisation by bacterium Azospirillum brasilense

Released on 10 April 2014, last updated on 21 May 2014
Triticum aestivum
Samples (4)
Protocols (7)
Plant growth promoting bacteria (PGPB) might be an alternative to increase nitrogenous use efficiency (NUE) in important crops such wheat. Azospirillum brasilense is one of the most promising PGPB and wheat roots colonized by Azospirillum brasilense is a good model to investigate the molecular basis of plant-PGPB interaction including improvement in plant-NUE promoted by PGPB. An RNA-seq transcriptional analysis of Triticum aestivum roots was carried out in two independent samples (biological replicates) of each treatment (PGPB-colonized or non-inoculated), yielding a total of 4 sequencing libraries, which were designated CWR1 and CWR2 libraries (colonized roots) and N-IWR1 and N-IWR2 (non-inoculated roots).
Experiment types
RNA-seq of coding RNA, stimulus or stress
Dual RNA-seq transcriptional analysis of wheat roots colonized by Azospirillum brasilense reveals up-regulation of nutrient acquisition and cell cycle genes. Doumit Camilios-Neto, Paloma Bonato, Roseli Wassem, Michelle Z Tadra-Sfeir, Liziane CC Brusamarello-Santos, Glaucio Valdameri, Lucélia Donatti, Helisson Faoro, Vinicius A Weiss, Leda S Chubatsu, Fábio O Pedrosa and Emanuel M Souza. BMC Genomics 15:378 (2014)
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-MTAB-2301.idf.txt
Sample and data relationshipE-MTAB-2301.sdrf.txt
Processed data (1)E-MTAB-2301.processed.1.zip