6 protocols
array scanning and feature extraction protocol
Agilent G2565CA Microarray Scanner System with Agilent Feature Extraction software (10.7)
nucleic acid hybridization to array protocol
Hybridization with Agilent Gene Expression Hybridisation Kit, incubated in Agilent SureHyb chambers for 17 hours at 65°C while rotating. Washing according to manufacturer’s instructions.
nucleic acid labeling protocol
300 ng of total RNA was labeled with Cy3 using the Agilent Quick-Amp Labeling Kit - 1 color according to the manufacturer´s instructions
nucleic acid extraction protocol
RNA isolated with RNeasy Mini Kit (Qiagen) according to manufacturer's instruction
treatment protocol
Loading with eLDL, oxLDL and deloading with HDL3. Loading: eLDL (40 ug/mL) or oxLDL (80 ug/mL);deloading: HDL3 (100 ug/mL)
growth protocol
1x10^6 cells/mL in macrophage serum-free medium (Invitrogen, Germany) supplemented with 50 ng/mL recombinant human monocyte-colony stimulating factor (M-CSF; R&D Systems, USA)