E-MTAB-2298 - Transcription profiling by array of monocyte to macrophage differentiation and loading/deloading with lipoproteins (eLDL, oxLDL, HDL3)
Submitted on 15 July 2010, released on 2 March 2014, last updated on 3 June 2014
Elutriated monocytes from 9 healthy donors with ApoE3/E3 genotype were differentiated for up to six days in vitro to macrophages using rhMCSF under serum-free conditions. Samples were taken on days 1, 4, 5 and 6 and analyzed for their transcriptomic profiles. After four days of differentiation cells were loaded for 24 hours with enzymatically modified LDL (eLDL, 40 µg/ml) or mildly oxidized LDL (oxLDL, 80 µg/ml). Finally on day five, lipid deloading was performed in serum free medium containing M-CSF and cells were incubated another 24 h with HDL3 (100 µg/mL).
transcription profiling by array, cell type comparison design, development or differentiation design, in vitro design
Monocyte to macrophage differentiation goes along with modulation of the plasmalogen pattern through transcriptional regulation. Stefan Wallner, Margot Grandl, Tatiana Konovalova, Alexander Sigrüner, Thomas Kopf, Markus Peer, Evelyn Orsó, Gerhard Liebisch, and Gerd Schmitz.
Monocyte to macrophage differentiation goes along with modulation of the plasmalogen pattern through transcriptional regulation. Wallner S, Grandl M, Konovalova T, Sigrüner A, Kopf T, Peer M, Orsó E, Liebisch G, Schmitz G. :e94102 (2014), Europe PMC 24714687