5 protocols
AccessionType
array scanning protocol
Target hybridization, staining, and scanning were performed according to the instructions designed for the Pseudomonas aeruginosa genome array by Affymetrix
nucleic acid hybridization to array protocol
Target hybridization, staining, and scanning were performed according to the instructions designed for the Pseudomonas aeruginosa genome array by Affymetrix
nucleic acid labeling protocol
cDNA synthesized from total RNA was fragmented and labeled using the Enzo BioArray terminal labeling kit
growth protocol
R. sphaeroides strain 2.4.1 (kindly provided by prof. Samuel Kaplan) and its mutants were grown aerobically at 30 ± 0.5�C in Sistrom minimal medium A containing succinate as a carbon source, as already described (Italiano et al., 2011). Sistrom medium was supplemented with CoCl2X6H2O (Carlo Erba, Italy) to a final 5 mM concentration, when required.
nucleic acid extraction protocol
Total RNA was isolated by the hot phenol procedure described in (Roh et al., 2004), including DNase and LiCl treatments, from R. sphaeroides 2.4.1 and mutant cells grown aerobically in Sistrom minimal medium (supplemented with 5mM Co2+ when required).