E-MTAB-2180 - Drosophila melanogaster 4C-Seq experiments in whole embro and mesoderm cells during embryogenesis
Released on 26 June 2014, last updated on 15 August 2014
Chromosome conformation capture (4C-Seq) in Drosophila Twist-H2B embryos (carrying nuclear tag specifically in the mesoderm) during embryogenesis was performed, anchoring on 107 different viewpoints. Two timepoints (3-4hrs and 6-8hrs after egg laying) and two tissue context (whole embryo and mesoderm) were assayed. Two independent collections were performed at each timepoint.
DNA-seq, development or differentiation design, organism part comparison design, replicate design
Enhancer loops appear stable during development and are associated with paused polymerase. Yad Ghavi-Helm, Felix A. Klein, Tibor Pakozdi, Lucia Ciglar, Daan Noordermeer, Wolfgang Huber & Eileen E. M. Furlong. Nature 512:96-100 (2014), PMID:25043061