E-MTAB-2148 - ChIP-seq of sigma 70 in wild-type and hns mutant Escherichia coli K-12 MG1655
Last updated on 15 December 2013, released on 15 December 2013
We mapped the genome-wide binding of sigma 70 in E. coli K-12 MG1655 and an hns mutant that is otherwise isogenic using ChIP coupled with deep sequencing (ChIP-seq). We show that intragenic binding of sigma 70 is increased in the hns mutant.
ChIP-seq, genotype design, in vivo
Widespread suppression of intragenic transcription initiation by H-NS. Singh, S.S., Singh, N., Bonocora, R.P., Fitzgerald, D.M., Wade, J.T. and Grainger, D.C.