E-MTAB-2138 - Transcription profiling by array of Saccharomyces cerevisiae to gain insight into the impact of THO complex sumoylation on gene expression
Released on 25 January 2014, last updated on 3 June 2014
To gain insight into the impact of THO complex sumoylation on gene expression in yeast S. cerevisiae, total RNAs were extracted from wt and hpr1-KR cells and transcriptome profiles were analyzed by Agilent microarrays. The hpr1 K-R mutant is a version of the hpr1 THO subunit in which lysines 60-65 were simultaneously mutated, which abrogated the sumoylation of the protein.
transcription profiling by array, co-expression, dye swap design, genetic modification design
Sumoylation of the THO complex regulates the biogenesis of a subset of mRNPs. Bretes H, Rouviere JO, Leger T, Oeffinger M, Devaux F, Doye V, Palancade B. :5043-5058 (2014), Europe PMC 24500206
Sumoylation of the THO complex is required for optimal mRNP metabolism. Hugo BRETES, Jerome ROUVIERE, Thibaut LEGER, Marlene OEFFINGER, Frederic DEVAUX, Valerie DOYE, Benoit PALANCADE.