E-MTAB-2072 - Transcription profiling by RNA-seq of human embryonic stem cells after RNAi knockdown of endogenous retrovirus HERVH
Released on 30 March 2014, last updated on 16 September 2019
Human endogenous retrovirus subfamily H (HERVH) is a class of transposable elements whose members are expressed preferentially in human pluripotent stem cells. Here, we report that the long-terminal repeat regions of HERVH function as enhancers which are regulated by OCT4. Strikingly, we found that HERVH is a nuclear-localized long non-coding RNA that is required to maintain the undifferentiated state of human embryonic stem cells. Furthermore, we showed that HERVH is associated with OCT4 and co-activators such as P300, CBP and mediator subunits. Together, these results uncover a new and unexpected role of species-specific transposable elements in specifying the pluripotency of human cells.
RNA-seq of coding RNA, cellular modification, co-expression, in vitro
The retrovirus HERVH is a long noncoding RNA required for human embryonic stem cell identity. Xinyi Lu, Friedrich Sachs, LeeAnn Ramsay, Pierre-Étienne Jacques, Jonathan Göke, Guillaume Bourque & Huck-Hui Ng.