E-MTAB-2072 - Transcription profiling by RNA-seq of human embryonic stem cells after RNAi knockdown of endogenous retrovirus HERVH

Status
Released on 30 March 2014, last updated on 16 September 2019
Organism
Homo sapiens
Samples (2)
Protocols (5)
Description
Human endogenous retrovirus subfamily H (HERVH) is a class of transposable elements whose members are expressed preferentially in human pluripotent stem cells. Here, we report that the long-terminal repeat regions of HERVH function as enhancers which are regulated by OCT4. Strikingly, we found that HERVH is a nuclear-localized long non-coding RNA that is required to maintain the undifferentiated state of human embryonic stem cells. Furthermore, we showed that HERVH is associated with OCT4 and co-activators such as P300, CBP and mediator subunits. Together, these results uncover a new and unexpected role of species-specific transposable elements in specifying the pluripotency of human cells.
Experiment types
RNA-seq of coding RNA, cellular modification, co-expression, in vitro
Contacts
Citations
The retrovirus HERVH is a long noncoding RNA required for human embryonic stem cell identity. Xinyi Lu, Friedrich Sachs, LeeAnn Ramsay, Pierre-Étienne Jacques, Jonathan Göke, Guillaume Bourque & Huck-Hui Ng.
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-MTAB-2072.idf.txt
Sample and data relationshipE-MTAB-2072.sdrf.txt
Links