E-MTAB-2042 - ChIP-Seq of STAT3 in 3iL Human Embryonic Stem Cells

Last updated on 27 November 2013, released on 5 December 2013
Homo sapiens
Samples (1)
Protocols (5)
Human embryonic stem cells (hESCs) are isolated from the inner cell mass of the blastocysts. The pluripotent properties of hESCs enable the derivation of cell-types or tissues of different lineages for potential applications such as therapeutics discovery and regenerative medicine. Even though hESCs are pluripotent, differences have been observed when compared to the native pluripotent epiblast cells of the blastocyst. We use a chemical approach (3iL: 3 small molecule inhibitor and cytokine) to induce an expression signature that more closely resembles native pluripotent cells. This experiment is STAT3 binding data in 3iL hESCs.
Experiment types
ChIP-seq, ChiP-seq, cell type comparison, in vitro
A LIF-dependent human pluripotent state with a distinctive regulatory circuitry. Yun-Shen Chan, Jonathan Goke, Jia-hui Ng, Xinyi Lu, Cheng-Peow Tan, Wei-Quan Tng, Zhong-Zhi Hong, Huck-Hui Ng.
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-MTAB-2042.idf.txt
Sample and data relationshipE-MTAB-2042.sdrf.txt