7 protocols
AccessionType
bioassay_data_transformation
Beadlevel data was processed using the beadarray and lumi packages in R and normalized using the robust spline method (RSN).
bioassay_data_transformation
Beadlevel data was processed using the beadarray and lumi packages in R and transformed using the variance-stabilizing transformation (VST).
image_acquisition
Chips were washed, detected and scanned according to the manufacturers instructions.
labeling
Total Prep RNA Amplification Kit (Illumina).
hybridization
1500ng of biotinylated RNA was hybridised to Illumina Mouse-6 v1.1 Expression Beadchips overnight at 58¼C.
grow
To begin to define the role of miR-22 in the mouse, we established miR-22-null mice. To do this, we induced homologous recombination using a gene replacement targeting vector to remove the miR-22 hairpin precursor. A mutant line containing the miR-22Brd-m2 (miR-22+/-) allele without the selection cassette was established in a pure 129S5/S7 genetic background for analysis.
nucleic acid extraction protocol
Total RNA was extracted from adult tissues using the miRNeasy protocol (Qiagen).