E-MTAB-2034 - RNA-seq of coding RNA from TCF7L2 knockdown and control INS1 832/13 cells to identify the downstream target genes that might be involved in the development of type 2 diabetes
Last updated on 7 November 2013, released on 15 December 2013
Genetic variants in TCF7L2 are the most highly associated variants with type 2 diabetes.We performed RNA_seq experiments on Control and Knockdown samples for 4 passages each. Library preparation was performed according to mRNA Sequening Sample Preparation Guide (illumina). Reagents were taken from the Illumina sample preparation kit v.2. Knockdown and control samples were sequenced together in one flowcell, 6 samples per lane. The aim of this study is to identify the downstream target genes that might the involvement of TCF7L2 in the development of type 2 diabetes. No technical replicate, 4 biological replicates.
RNA-seq of coding RNA, cellular modification design, co-expression
TCF7L2 is the master regulator of insulin production and processing. Yuedan Zhou, Soo-Young Park, Jing Su, Kathleen Bailey,Enming Zhang,Thomas Thevenin,Liliya Shcherbina,Emilia Ottosson-Laakso,Emma Ahlqvist,Johan Rung,Claes Wolheim,Marcelo Nobrega,Erik Renstr_m,Leif Groop,Ola Hansson.