E-MTAB-2031 - RNA-Seq of 3iL human embryonic stem cells with an induced expression profile that more closely resembles native pluripotent cells and uninduced controls
Released on 12 December 2014, last updated on 26 April 2016
Human embryonic stem cells (hESCs) are isolated from the inner cell mass of the blastocysts. The pluripotent properties of hESCs enable the derivation of cell-types or tissues of different lineages for potential applications such as therapeutics discovery and regenerative medicine. Even though hESCs are pluripotent, differences have been observed when compared to the native pluripotent epiblast cells of the blastocyst. We use a chemical approach (3iL: 3 small molecule inhibitor and cytokine) to induce an expression signature that more closely resembles native pluripotent cells. To better understand the difference between this hESC state and the conventional hESC state, we generated the global expression profiles using RNA-seq.
RNA-seq of coding RNA, cell type comparison, co-expression, in vitro
Induction of a human pluripotent state with distinct regulatory circuitry that resembles preimplantation epiblast. Chan YS, Göke J, Ng JH, Lu X, Gonzales KA, Tan CP, Tng WQ, Hong ZZ, Lim YS, Ng HH. , Europe PMC 24315441