nucleic acid sequencing protocol
Fragments were sequenced using the Illumina HiSeq 2000
nucleic acid extraction protocol
In vitro human pancreatic progenitor cells were fixed, sonicated and processed for ChIP as described (van Arensbergen, J. et al. Genome Res 20:722, 2010) using 1-1.5ug of the following antibodies: rabbit anti-H3K4me1 (ab-8895), goat anti-Pdx1 (Chris Wright, BCBC AB2027), goat anti-FOXA2 (sc-6554), rabbit anti-GATA6 (sc-9055X), rabbit anti-HNF1B(sc-22840-X), rabbit anti-ONECUT1 (sc-13050).
The differentiation protocol is described in Cho, C.H. et al. Inhibition of activin/nodal signalling is necessary for pancreatic differentiation of human pluripotent stem cells. Diabetologia 55, 3284-3295 (2012)
nucleic acid library construction protocol
The immunoprecipitated DNA was modified for sequencing following the Illumina sequencing following the manufacturer protocol (Preparing Sample for ChIP Sequencing of DNA (11257047 Rev A)).