6 protocols
normalization data transformation protocol
The scanned images were analyzed with Feature Extraction Software 10.5 (Agilent) using default parameters.
array scanning protocol
As per manufacturer (Agilent)
nucleic acid extraction protocol
Total RNA was extracted from fresh frozen tissues using RNeasy kit (Qiagen) according to the manufacturer's instructions including on-column DNase digestion. Total RNA was checked by the 2100 Agilent BioAnalyzer System (Agilent Technologies) to check for possible contamination and degradation.
nucleic acid hybridization to array protocol
As per manufacturer (Agilent)
nucleic acid labeling protocol
As per manufacturer (Agilent)
growth protocol
Surgical specimens were stored in liquid nitrogen