E-MTAB-1969 - microRNA profiling by high throughput sequencing of miRNAs that are differentially regulated during maturation of monocytes and polarization of macrophages by cytokines
Released on 14 January 2014, last updated on 2 May 2014
microRNAs are small non-coding RNAs that regulate gene expression at a post-translational level, and play a crucial role in the development of cells of the immune system. Macrophages are essential for generating inflammatory reactions upon tissue damage and encountering of invading pathogens, yet modulation of their immune responses is critical for maintaining tissue homeostasis. Macrophages can present different phenotypes, depending on the cytokine environment they encounter in the affected tissues. In this study, we have identified expression signatures of miRNAs that are differentially regulated during maturation of monocytes and polarization of macrophages by cytokines. We present a comprehensive characterization of miRNA expression in human monocytes and M1, M2a and M2c polarized macrophages, using next-generation Sequencing by Oligonucleotide Ligation and Detection (SOLiD). We have analyzed freshly isolated human monocytes and 5 day monocyte-derived macrophages unstimulated, or stimulated with IFNgamma+TNFalpha (M1), IL-4 (M2a) or IL-10 (M2c).
RNA-seq of non coding RNA, cell type comparison design, growth condition design, in vitro, microRNA profiling by high throughput sequencing
Next-generation sequencing of microRNAs in primary human polarized macrophages. Cobos Jiménez V, Willemsen AM, Bradley EJ, Baas F, van Kampen AH, Kootstra NA. :181-183 (2014), Europe PMC 26484091
Next-generation sequencing of microRNAs uncovers expression signatures in polarized macrophages. Viviana Cobos Jiménez, Edward J. Bradley, Antonius M. Willemsen, Antoine H.C. van Kampen, Frank Baas and Neeltje A. Kootstra. Physiological Genomics (2013), Europe PMC 24326348