E-MTAB-1888 - Transcription profiling by array of rat vascular smooth muscle cells (VSMCs) grown on a micro-patterned groove substrate
Released on 14 November 2013, last updated on 2 May 2014
Loss of contractility and acquisition of an epithelial phenotype of vascular smooth muscle cells (VSMCs) are key events in proliferative vascular pathologies such as atherosclerosis and post-angioplastic restenosis. There is no proper cell culture system allowing VSMC differentiation so that it is difficult to delineate the molecular mechanism responsible for proliferative vasculopathy. We investigated whether a micro-patterned substrate could restore the contractile phenotype of VSMCs in vitro. To induce and maintain the differentiated VSMC phenotype in vitro, we introduced a micro-patterned groove substrate to modulate the morphology and function of VSMCs.
transcription profiling by array, co-expression, growth condition design, in vitro
Phenotypic modulation of primary vascular smooth muscle cells by short-term culture on micropatterned substrate. Chang S, Song S, Lee J, Yoon J, Park J, Choi S, Park JK, Choi K, Choi C. PLoS One 9(2):e88089 (2014), Europe PMC 24505388