E-MTAB-1867 - RNA-seq of coding RNA for the European seabass, Dicentrarchus labrax, from two targeted organs
Last updated on 16 September 2013, released on 1 February 2014
We aimed at obtaining a reference transcriptome for the European seabass. We characterized by Illumina paired-ends RNA-seq the D. labrax transcriptome for two targeted organs, using a diversity of conditions, animal stages and genotypes to warranty the widest variety of reconstructed transcripts. The brain (including pineal gland and pituitary) and liver were chosen as they are complementary and at a crossroads of many key neuroendocrine, metabolic and behavioral regulations. Dicentrarchus labrax_2 targeted organs (liver and brain) with two technical replicates each (using two different fragment sizes) half a GAIIX run total. We used two flow cells per organ and each was corresponding to a different fragment size. The expected sizes were 350 and 500bp.
RNA-seq of coding RNA, organism part comparison design
Generation and Characterization of the sea bass Dicentrarchus labrax transcriptome. Elodie Magnanou, Christophe Klopp, Celine Noirot, Laurence Besseau, Jack Falcón.