nucleic acid labeling protocol
Labeling of samples was carried out according to the manufacturer’s standard protocol
nucleic acid hybridization to array protocol
P-AFFY-3 GeneChip hybridization
normalization data transformation protocol
After 8 weeks of chronic mild stress rats were decapitated, brain removed and snap frozen. Then sliced in 50um and Lateral habenullas were microdeissected.
During last 4 weeks of CMS protocol some rats were antidepressant treated with selective serotonin reuptake inhibitor Escitalopram (5 mg/kg/day) and saline as vehicle
nucleic acid extraction protocol
Total RNA was isolated from each sample by use of miRNeasy Mini Kit (Qiagen Inc., USA) according to the manufacturers protocol.