5 protocols
normalization data transformation protocol
The data were normalized by the Loess method using the limma (Linear Models for Microarray Data) package in R. Statistical analysis of the microarray data was performed using the significance of microarrays (SAM)package
array scanning protocol
Arrays were scanned with the Agilent Technologies Scanner G2505C according to manufacturer's specification
nucleic acid hybridization to array protocol
Labelled cRNA was hybridized using Agilent hybridization chamber and oven as recommended by the manufacturer(Parameters: Chamber type = OTHER: Agilent chamber, Quantity of label target used = 825, Mass unit = Nano gram, time = 17, Tiny time unit = hours, Volume = 100, Volume unit = Micro litre, temperature = 65)
nucleic acid labeling protocol
Five-hundred nanograms of total RNA of each sample were amplified and labeled using the Quick Amp Labeling Kit (Agilent Technologies)
nucleic acid extraction protocol
liver tissue was homogenized in Trizol. Following chlorophorm extraction, 1 volume 70% EtOH was added to the aqueous phase, and RNA was extracted using the RNeasy kit (Qiagen)