E-MTAB-1799 - Transcription profiling by array of Solanum tuberosum Neo-Tuberosum and Solanum tuberosum ssp. Andigena to investigate day length dependent re-structuring of the leaf transcriptome and metabolome in potatoes with contrasting tuberisation phenotypes
Released on 1 November 2013, last updated on 3 June 2014
Recent advances have defined some of the components of photoperiodic signalling that lead to tuberisation in potato including orthologues of FLOWERING LOCUS T (StSP6A) and CYCLING DOF FACTOR (StCDF1). The aim of the current study is to investigate the molecular basis of permissive tuber initiation under long days in Solanum tuberosum Neo-Tuberosum by comparative analysis with an obligate short day Solanum tuberosum ssp. Andigena accession. We show that the Neo-Tuberosum accession, but not the Andigena, contains alleles that encode StCDF1 proteins modified in the C-terminal region, likely to evade long day inhibition of StSP6A expression. We also identify an allele of StSP6A from the Neo-Tuberosum accession, absent in the Andigena, which is expressed under long days. Other leaf transcripts and metabolites that show different abundances in tuberising and non-tuberising samples were identified adding detail to tuberisation-associated processes. Overall, the data presented in this study highlight the subtle interplay between components of the clock-CONSTANS-StSP6A axis which collectively may interact to fine-tune the timing of tuberisation.
transcription profiling by array, co-expression, growth condition design, stimulus or stress design, time series design
Day length dependent re-structuring of the leaf transcriptome and metabolome in potatoes with contrasting tuberisation phenotypes. Wayne L. Morris, Robert D. Hancock, Laurence J.D. Ducreux, Jenny A. Morris, Muhammad Usman, Susan R. Verrall, Glenn Bryan, James W. McNicol, Pete E. Hedley, Mark A. Taylor.