7 protocols
normalization data transformation protocol
The software tools Feature Extraction, GeneSpring GX 11.5.1 and Spotfire Decision Site 9.1.2 (TIBCO) were used for quality control and statistical data analysis
array scanning protocol
Fluorescent signal intensities were detected with Scan Control A.8.4.1 software (Agilent Technologies) in the Agilent DNA microarray scanner and extracted from the images using Feature Extraction software (Agilent Technologies)
nucleic acid hybridization to array protocol
A one-colour based hybridisation protocol was performed at 65C for 17 hours on separate mouse GE v2 microarray platforms (4x44K format) (G4846A, Agilent Technologies)
nucleic acid labeling protocol
100ng of total RNA per sample was reverse transcribed into cDNA and then converted into labelled cRNA by in vitro transcription incorporating cyanine-3-CTP (Low input quick-amp labelling kit one-colour, Agilent Technologies)
growth protocol
Heterozygous matings of Splotch-delayed mice were harvested at E14.5 (TS23)
treatment protocol
Splotch-delayed homozogous mutant and control (homozygous wild-type and heterozygote) embryos were dissected and Humerus forelimb tissue was dissected.
nucleic acid extraction protocol
RNA was extracted following manufacturers guidlines ( SV. Total RNA Isolation system, Promega, UK)