nucleic acid sequencing protocol
Illumina 76bp paired-end reads
nucleic acid library construction protocol
Illumina mRNA-seq library construction protocol.
nucleic acid extraction protocol
C. elegans strain PS1010 was grown on nutrient agar (Difco) supplemented with 0.1% v/v cholesterol in the presence of E. coli HB101.
Isogenic (y1; cn bw1 sp1) embryos were cultured on standard Drosophila medium.
Cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin.