E-MTAB-1715 - RNA-seq of coding RNA in bloodstream form of Trypanosoma brucei with or without depletion of histone H1 by RNA intereference to study if histone H1 is involved in transcription regulation
Last updated on 4 July 2014, released on 10 July 2014
Trypanosoma brucei brucei
Our goal was to determine if histone H1 is involved in transcription regulation. RNA interference was used to deplete histone H1 from bloodstream form T. brucei in culture. Total RNA was extracted and RNAseq was used to compare the expression profile of H1 depleted clones and parental cell-line.
RNA-seq of coding RNA, biological variation design, cellular modification design
Trypanosoma brucei histone H1 inhibits RNA Polymerase I transcription and is important for parasite fitness in vivo. Ana C. Pena, Mafalda R. Pimentel, Helena Manso, Rita Vaz-Drago, Daniel Neves, Francisco Aresta-Branco, Filipa R. Ferreira, Fabien Guegan, Luis Pedro Coelho, Maria Carmo-Fonseca, Nuno L. Barbosa-Morais, Luisa M. Figueiredo.