6 protocols
normalization data transformation protocol
quantile normalization using bioconductor package lumi, log2 transformation
array scanning protocol
BeadArray Reader S376
nucleic acid hybridization to array protocol
cRNA was hybridized to Illumina HumanHT-12 v3 Expression BeadChip
nucleic acid labeling protocol
RNA was reverse transcribed and biotin-UTP-labeled into cRNA using the Illumina TotalPrep-96 RNA Amplification kit
growth protocol
human whole blood collected under fasting conditions as part of the KORA F4 study. “KORA” stands for “The Cooperative Health Research in the Region of Augsburg”. KORA surveys are population-based studies (Holle et al., 2005)*. In survey number 4 (S4) 4261 individuals were examined from 1999 to 2001. A first follow-up (F4) survey was conducted from 2006 to 2008 with 3080 participants from the original S4 study took part in F4. See more in http://www.helmholtz-muenchen.de/en/kora-en/information-for-scientists/current-kora-studies/f4-study/index.html . * Holle R, Happich M, Lowel H, Wichmann HE (2005) KORA - a research platform for population based health research. Gesundheitswesen 67: S19-25
nucleic acid extraction protocol
RNA extraction with PAXgene (BD) Blood miRNA kit (Qiagen)