E-MTAB-1704 - RNA-seq of coding RNA of brown adipose tissue from Ehmt1flox/flox control mice, Ehmt1Myf5 Knockout mice and Ehmt1adipo knockout mice to study the role of euchromatic histone-lysine N-methyltransferase 1 (EHMT1)
Released on 7 November 2013, last updated on 2 May 2014
Brown adipose tissue (BAT) dissipates chemical energy in the form of heat, as a defense against hypothermia and obesity. Current evidence indicates that brown adipocytes arise from Myf5+-dermotomal precursors through the action of a PRDM16-C/EBP-_ transcriptional complex; however, the underlying mechanisms that determine lineage specification and maintenance of brown adipose cells remain poorly understood. Here we study the role of euchromatic histone-lysine N-methyltransferase 1 (EHMT1), a brown fat-enriched lysine methyltransferase, as an essential enzymatic component of the PRDM16 transcriptional complex and controls brown adipose cell fate. To identify targets and function of EHMT1, we performed genome-wide gene expression profiling of BAT from control mouce (Ehmt1flox/flox), Ehmt1Myf5 KO mouse (Myf5-Cre+/-; Ehmt1flox/flox) and Ehmt1adipo KO mouse (Adipo-Cre+/-; Ehmt1flox/flox). Loss of EHMT1 in Myf5+ lineage causes a near total loss of brown fat characteristics and induces muscle-selective gene program in vivo. In addition, adipose-specific deletion of EHMT1 by Adipo-Cre leads to a marked reduction of the thermogenic and fat oxidation genes.
RNA-seq of coding RNA, co-expression, in vivo, individual genetic characteristics
EHMT1 controls brown adipose cell fate and thermogenesis through the PRDM16 complex. Ohno H, Shinoda K, Ohyama K, Sharp LZ, Kajimura S. :163-167 (2013), Europe PMC 24196706
EHMT1 controls brown adipose cell fate and thermogenesis through the PRDM16 complex. Haruya Ohno, Kosaku Shinoda, Kana Ohyama, Louis Z. Sharp and Shingo Kajimura. Nature (2013)