E-MTAB-1685 - ChIP-chip by array using chromatin from rosette leaves of 35S:H3.3-YFP Arabidopsis thaliana plants in order to profile H3.3, a major H3 histone variant
Released on 10 March 2014, last updated on 26 June 2014
Histone variants can effect nucleosome stability or affect histone of DNA modifications. H3.3 is a major H3 histone variant that is incorporated into chromatin outside of S-phase in various eukaryotes. In animals, H3.3 is associated with active transcription and possibly maintenance of transcriptional memory. Plant H3.3, which evolved independently of animal H3.3, is much less well understood. We performed ChIP-chip using chromatin from rosette leaves of 35S:H3.3-YFP plants.
ChIP-chip by array, binding site identification design, replicate
Arabidopsis replacement histone variant H3.3 occupies promoters of regulated genes. Shu H, Nakamura M, Siretskiy A, Borghi L, Moraes I, Wildhaber T, Gruissem W, Hennig L. Genome Biol 15(4):r62 (2014), Europe PMC 24708891