E-MTAB-1678 - transcriptome of holcus lanatus
Released on 4 November 2013, last updated on 3 May 2014
Holcus lanatus L
The wild grass Holcus lanatus L., an outcrossing diploid (2n=14) and closely related to B. distachyon (Aliscioni et al., 2012), has a remarkable balanced polymorphism in arsenate tolerance, screened from a semi-natural, non-arsenic contaminated populations (Meharg et al., 1993), coded by a single gene (Macnair et al., 1992). As arsenate is a phosphate analogue it has been postulated that this polymorphism is maintained due to phosphorus nutrition, not arsenate tolerance per se, particularly as the tolerance gene co-segregates with suppression of High affinity Phosphate Transport (HAPT) (Meharg et al., 1992a; Meharg & Macnair, 1992b), though an explicit ecological link to phosphorus status of soils has yet to be proven (Naylor et al., 1996). The aim of this study is to address soil phosphate responsiveness (+/-) along with the transcriptomic consequences of being of arsenic tolerant (T) or non-tolerant (N) phenotype to ascertain why and how this polymorphism is maintained.
RNA-seq of coding RNA, co-expression, stimulus or stress
Trait-directed de novo population transcriptome dissects genetic regulation of a balanced polymorphism in phosphorus nutrition/arsenate tolerance in a wild grass, Holcus lanatus. Meharg C, Khan B, Norton G, Deacon C, Johnson D, Reinhardt R, Huettel B, Meharg AA. New Phytol (2013), Europe PMC 24102375