11 protocols
AccessionType
normalization data transformation protocol
GenomeStudio Data Analysis Software (Illumina, Inc.) J-express 2009 (Molmine AS)
array scanning protocol
iScan system/reader, Illumina
nucleic acid hybridization to array protocol
Whole - Genome Gene Expression Direct Hybridization Assay Guide, Illumina inc
nucleic acid labeling protocol
nucleic acid extraction protocol
Total RNA from MCF-7 cells were extracted using RNeasy Mini kit (Qiagen, CA). http://www.genome.duke.edu/cores/microarray/services/rna-qc/documents/RNeasy_Mini_Handbook.pdf
growth protocol
MCF-7 human breast cancer cells transduced with shRNA targeting SRC-2 (SRC-2 shRNA) or infected with control shRNA vector (Ctr shRNA) were seeded in 9,2 cm petri dishes, 2,0 x106 cells in each dishes, in DMEM supplemented with 5% charcoaled stripped FBS and 10 nM 17b-estradiol.
treatment protocol
Cells were treated with 10 nM 17b-estradiol for 3 days
treatment protocol
Cells were treated with 10 nM 17b-estradiol for 3 days
growth protocol
MCF-7 human breast cancer cells transduced with shRNA targeting SRC-2 (SRC-2 shRNA) were seeded in 9,2 cm petri dishes, 2,0 x106 cells in each dishes, in DMEM supplemented with 5% charcoaled stripped FBS and 10 nM 17b-estradiol.
growth protocol
MCF-7 human breast cancer cells transduced with control shRNA vector (Ctr shRNA) were seeded in 9,2 cm petri dishes, 2,0 x106 cells in each dishes, in DMEM supplemented with 5% charcoaled stripped FBS and 10 nM 17b-estradiol.
treatment protocol
The cells were treated with 10 nM 17b-estradiol for 48. After 48 hours the Ctr shRNA cells were treated with cAMP analog (8-CPT-cAMP, 150 µM) and cAMP elevating agents (Forskolin (10 µM) and IBMX (50 µM)) to activate the cAMP/PKA signaling pathway. The cells were then incubated for another 24 hour in the cell incubator.