6 protocols
normalization data transformation protocol
read count normalization; factor calculated by (number of reads mapped to miRNA / 1 million)
high throughput sequence alignment protocol
novoalign v2.08.01; parameter: -a -l 15 -A ; reference genome hg19
nucleic acid sequencing protocol
TruSeq SBS v2; 36 bp single end
nucleic acid extraction protocol
Extraction of total RNA with TriReagent (Ambion) following manufacturers protocol
growth protocol
The samples were collected from the main surgical specimen at the time of surgery for colorectal cancer, and immediately snap frozen in liquid nitrogen.
nucleic acid library construction protocol
Following Illumina`s TruSeq smallRNA protocol; starting with 1 ug total RNA